Cytotoxic (brine Shrimp Lethality Bioassay ) and Antioxidant Investigation of Barringtonia Acutangula (l.)”

Barringtonia acutangula (L.) gaertn. (Family: Barringtoniaceae), a medicinal small to medium evergreen tree known as ‘Hijal’, is used in diarrhoea, dysentery, colic , flatulence, cooling, aperients expectorant, stimulating emetic, astringents to the bowel, antihelminthic, bronchitis, lumber pain, hallucinations, seminal weakness, gonorrhoea and many other ailments in rural areas of Bangladesh It is also used as traditional medicine in other countries. But till to date, sporadic attempts have been made for the scientific and methodical validation of these traditional claims. In Brine Shrimp Lethality Bioassay, all the extracts produced dose dependent cytotoxicity effect to brine shrimp nauplii with methanol extract of leaf exhibiting highest toxicity having LC50 value 46.24 μg/ml where standard vincristine sulphate had the LC50 value of 0.69 μg/ml. & In antioxidant attempt by reducing power and CUPRAC assays, pet. ether extract of leaf were found to exhibit moderate but concentration dependent reducing power respectively. Key Word: sporadic; hallucinations; IC50 value; ascorbic acid, Vincristine sulphate Introduction Barringtonia acutangula (L) Geartn is distributed throughout the country. But it is mainly found in Chittagong, Chittagong Hill Tracts, Tangail, Sylhet and Dhaka. (Ghani A., 2003) Barringtonia acutangula (L) Geartn the plant selected for the current study, has various ethnopharmacological use and a tuberous herb from (Family: Barringtoniaceae). The family of (Family: Barringtoniaceae) has some medicinally valuable species. Barringtonia acutangula is a neglected species on which very few scientific investigations have been conducted. There remains a possibility that the extract of the plant may possess some bioactive compounds. The work described in this article is dedicated to phytochemically and pharmacologically characterize different parts of the specified plant to: (1) Identify the groups of chemical constituents present in the plant parts (2) Rationalize the traditional uses of the selected plant (3) Explore the possible newer medicinal activities of the same plant those are not traditionally claimed However, in order to develop these medicinal plants as drugs, attempts should be first made to certainly identify them and preclinical studies on them should be carried out to establish their claimed therapeutic properties. These are very important because the biological activity of a plant or its preparation will assist on determining the therapeutic target of its development. Since the chemical constituents and pharmacological actions of most of these plants are known and as they are in current use in traditional medicines, their clinical evaluation can be undertaken. The present study was designed to identify the groups of chemical constituents that are present in the crude extracts of Barringtonia acutangula (L) Gearth as well as to observe the pharmacological activities of the extracts of the plant. The present study was designed to identify the groups of chemical constituents that are present in the crude extracts of Barringtonia acutangula (L) Gearth as well as to observe the pharmacological activities of extracts of the plant parts of leaves & bark with the solvent of Petroleum ether & Methanol. Brine Shrimp lethality bioassay tests to find out the presence of chemical that has a cytotoxic effects on cell line. Antioxidant activity tests to find out Rerducing power & CUPRAC Assay. Md. Asaduzzaman et al. / International Journal of Pharma Sciences and Research (IJPSR) ISSN : 0975-9492 Vol. 6 No.8 Aug 2015 1179